Regulatory

Part:BBa_K2569025:Design

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-09-29)

Prib, constitutive promoter in Pseudomonas. fluorescence pf-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promoter Prib is a strong constitutive promoter that can be used in regulating the gene expression in Pseudomonadaceae.

Source

Its sequence is taken from the upsteam of the gene rib by artificially sythesis.

References